Inoculation and colonization of isolated entomopathogenic fungi Beauveria bassiana in rice plants, Oryza sativa L. through seed immersion method

Abstract Background The aims of the present study were to isolate and identify native entomopathogenic fungi, Beauveria bassiana from Egyptian soil evaluate artificial establishment B . as endophytes in rice plants through seeds immersion. Results Ten samples collected different cultivated fields at Ismailia Governorate. Only one sample was positive with a ratio 10%. Sequencing internal transcribed spacer (ITS) region indicated that obtained belongs registered under accession no. MN337282. To test endophytic colonization , soaked by concentration 5 × 10 7 spores/ml, when become an endophyte plants. examined for presence 30 days post treatment. PCR amplification using fungal specific primers conserved β-tubulin gene yielded identical 360 bp products both treated Conclusion results showed immersion conidial suspension proved be good method introduce into leaves protect against stem borers.